CommaPrep™ plasmid maxiprep columns are supplied with spin columns and collection tubes.The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 500 μg (NP500) or 1.5 mg (HP1000).
Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.
Features
- Up to 500 μg-1.5 mg high purity plasmid DNA
- Simple procedures and easy to operate
- Suitable for various downstream applications
Typical results
Table 1: The yield of plasmid DNA extracted using various columns
| 1 |
A260/280 |
Concentration（ng/μL） |
Yield（μg） |
| NP500 |
1.95 |
300 |
300 |
| 1 |
1.91 |
337 |
320 |
| HP1000 |
2.01 |
780 |
780 |
| 1 |
1.98 |
800 |
800 |
pUC19 was extracted from 120 mL overnight E.coli (DH5a) using columns NP500 HP1000,and the elution volume was 1.0 mL.
Experimental overview
Order information:
| Cat. # |
Description |
Volume |
Yield |
Qty. |
| NP500 |
CommaPrep™ Plasmid Maxiprep Columns |
50 mL，22 mL |
~500 μg |
20/Box |
| HP1000 |
CommaPrep™ High-yield Plasmid Maxiprep Columns |
50 mL，22 mL |
~1.5 mg |
20/Box |
| MNP001-4 |
CommaX™ Plasmid Maxiprep Kits |
1 |
1 |
10 Preps |
Note: It is recommended that the bacteria culture should be 100-200 mL and the elution volume should be 1.0-2.0 mL by using CommaPrepTM plasmid maxiprep columns.
