Product Description

CommaPrep™ plasmid maxiprep columns are supplied with spin columns and collection tubes.The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 500 μg (NP500) or 1.5 mg (HP1000).

Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.

Features

• Up to 500 μg-1.5 mg high purity plasmid DNA
• Simple procedures and easy to operate
• Suitable for various downstream applications

Typical results

Table 1: The yield of plasmid DNA extracted using various columns

1	A260/280	Concentration（ng/μL）	Yield（μg）
NP500	1.95	300	300
1	1.91	337	320
HP1000	2.01	780	780
1	1.98	800	800

pUC19 was extracted from 120 mL overnight E.coli (DH5a) using columns NP500 HP1000,and the elution volume was 1.0 mL.

 

Experimental overview

Order information:

Cat. #	Description	Volume	Yield	Qty.
NP500	CommaPrep™ Plasmid Maxiprep Columns	50 mL，22 mL	~500 μg	20/Box
HP1000	CommaPrep™ High-yield Plasmid Maxiprep Columns	50 mL，22 mL	~1.5 mg	20/Box
MNP001-4	CommaX™ Plasmid Maxiprep Kits	1	1	10 Preps

Note: It is recommended that the bacteria culture should be 100-200 mL and the elution volume should be 1.0-2.0 mL by using CommaPrepTM plasmid maxiprep columns.

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